Regrettably, the most severe cases are characterized by an insufficiency of donor sites. Although cultured epithelial autografts and spray-on skin treatments permit the application of smaller donor tissues, thereby alleviating donor site morbidity, they present their own challenges, notably in maintaining tissue integrity and precisely controlling cell placement. Researchers are investigating the potential of bioprinting to fabricate skin grafts, a process that depends significantly on several factors, including the efficacy of bioinks, the nature of the cells being used, and the ease of printing. This work explores a collagen-based bioink, permitting the placement of a continuous sheet of keratinocytes directly onto the wound. The intended clinical workflow was a key element of special attention. Since alterations to the media are impossible following bioink placement on the patient, we first formulated a media solution enabling a single deposition procedure, thereby promoting cellular self-assembly into the epidermis. Our immunofluorescence study of an epidermis grown from a collagen-based dermal template containing dermal fibroblasts, demonstrated the presence of markers typical of natural skin, including p63 (stem cell marker), Ki67 and keratin 14 (proliferation markers), filaggrin and keratin 10 (keratinocyte differentiation and barrier function markers), and collagen type IV (basement membrane protein facilitating epidermal-dermal adhesion). To fully verify its application in treating burns, additional tests are warranted, but our existing results suggest the potential of our current protocol to yield a donor-specific model for testing purposes.
The technique of three-dimensional printing (3DP) displays versatile potential for materials processing in the fields of tissue engineering and regenerative medicine, proving popular. The repair and rebuilding of considerable bone voids remain substantial obstacles in clinical practice, necessitating biomaterial implants to uphold mechanical strength and porosity, an aim potentially facilitated by 3DP techniques. A bibliometric survey of the past decade's evolution in 3DP technology is critical for identifying its applications in bone tissue engineering (BTE). This comparative study, which used bibliometric methods, focused on 3DP's applications within the domain of bone repair and regeneration. A comprehensive review of 2025 articles unveiled a noticeable rise in global 3DP publications and research interest over the preceding years. In this field, China spearheaded international cooperation, simultaneously emerging as the most prolific contributor in terms of cited publications. The overwhelming number of articles pertaining to this subject area appeared in the journal, Biofabrication. Chen Y's authorship is responsible for the most considerable contribution within the included studies. electrodiagnostic medicine The publications' content primarily focused on bone regeneration and repair, using keywords revolving around BTE and regenerative medicine, which further included 3DP techniques, 3DP materials, bone regeneration strategies, and bone disease therapeutics. A bibliometric and visualized examination of the evolution of 3DP in BTE from 2012 to 2022 offers significant insights, benefiting scientists in their pursuit of further investigation in this dynamic area.
The expanding realm of biomaterials and printing technologies has unlocked significant bioprinting potential for fabricating biomimetic architectures and living tissue models. Machine learning (ML) is implemented to provide greater potency to bioprinting and bioprinted constructs, optimizing associated processes, applied materials, and resulting mechanical and biological characteristics. We sought to collate, analyze, categorize, and summarize relevant articles and papers on the use of machine learning in bioprinting and its effect on the characteristics of bioprinted structures, as well as future prospects. Leveraging the accessible information, both traditional machine learning and deep learning approaches have been successfully applied to refine printing procedures, enhance structural features, improve the qualities of the materials, and optimize the biological and mechanical properties of bioprinted structures. Prediction models constructed using the former approach rely on features extracted from images or numerical information, while the latter models utilize the image itself for tasks like segmentation or classification. These studies employ advanced bioprinting technologies, exhibiting a stable and reliable printing process, optimal fiber/droplet diameters, and precise layer-by-layer stacking, while concurrently enhancing the bioprinted constructs' design and cellular performance parameters. Developing process-material-performance models for bioprinting presents current challenges and future opportunities, offering a potential paradigm shift in bioprinted designs and technologies.
Spheroid fabrication using acoustic cell assembly devices is characterized by its rapid, label-free, and low-cell-damage methodology, resulting in the production of spheroids with uniform sizes. Despite the progress in spheroid creation and yield, the current production methods are insufficient to satisfy the demands of diverse biomedical applications, particularly those requiring substantial quantities of spheroids for tasks like high-throughput screening, macro-scale tissue engineering, and tissue regeneration. Using gelatin methacrylamide (GelMA) hydrogels in conjunction with a novel 3D acoustic cell assembly device, we successfully achieved high-throughput fabrication of cell spheroids. Osteogenic biomimetic porous scaffolds Three orthogonal piezoelectric transducers are integrated into the acoustic device to create three orthogonal standing bulk acoustic waves. The result is a 3D dot array (25 x 25 x 22) of levitated acoustic nodes, enabling large-scale cell aggregate fabrication, yielding over 13,000 per operation. The GelMA hydrogel scaffold is crucial for preserving the structure of cell aggregates when acoustic fields are removed. Subsequently, nearly all cell clusters (>90%) evolve into spheroids, preserving excellent cell viability. Drug testing was further conducted on these acoustically assembled spheroids to investigate their potency in drug response. In essence, this 3D acoustic cell assembly device's potential lies in its ability to scale up the production of cell spheroids or even organoids, thereby offering flexibility for use in various biomedical applications, such as high-throughput screening, disease modeling, tissue engineering, and regenerative medicine.
The utility of bioprinting extends far and wide, with substantial application potential across various scientific and biotechnological fields. Medical advancements in bioprinting are directed towards generating cells and tissues for skin restoration, and also towards producing usable human organs, such as hearts, kidneys, and bones. This review provides a historical perspective on important bioprinting developments and their current standing. A search encompassing the SCOPUS, Web of Science, and PubMed databases uncovered a total of 31,603 articles; following careful assessment, only 122 were deemed suitable for the subsequent analysis. In these articles, the significant medical breakthroughs, practical applications, and present-day possibilities of this technique are addressed. The paper concludes by providing perspectives on bioprinting's applications and our anticipated advancement in this technology. A review of bioprinting's remarkable advancement from 1998 to the present is presented in this paper, showcasing promising results that bring our society closer to fully reconstructing damaged tissues and organs, thereby addressing healthcare issues like the scarcity of organ and tissue donors.
Utilizing bioinks and biological factors, 3D bioprinting, a computer-managed process, crafts a precise three-dimensional (3D) structure in a layer-by-layer manner. 3D bioprinting, arising from rapid prototyping and additive manufacturing, a novel tissue engineering technology, also draws upon expertise from numerous diverse disciplines. Problems with the in vitro culture procedure extend to the bioprinting process, which itself is plagued by issues such as (1) the selection of a bioink that matches printing parameters to lessen cellular damage and death, and (2) the enhancement of printing precision. Powerful predictive capabilities inherent in data-driven machine learning algorithms provide natural advantages in exploring new models and predicting behavior. The integration of 3D bioprinting with machine learning algorithms aids in the development of improved bioinks, the precise determination of printing parameters, and the identification of printing faults. The paper presents a detailed description of various machine learning algorithms, highlighting their importance in additive manufacturing. It then summarizes the influence of machine learning on applications in additive manufacturing. Furthermore, this work reviews the research on integrating 3D bioprinting with machine learning, particularly with regard to advancements in bioink formulation, printing parameter adjustments, and the detection of printing anomalies.
Despite improvements in prosthetic materials, surgical techniques, and operating microscopes during the last fifty years, enduring hearing restoration remains a complex challenge in ossicular chain reconstruction procedures. The inadequacy of prosthesis length or shape, along with surgical procedure flaws, are the primary culprits behind reconstruction failures. A 3D-printed middle ear prosthesis holds promise for tailoring treatment and achieving superior outcomes for individual patients. This investigation sought to characterize the potential and limitations of employing 3D-printed middle ear replacements. A commercial titanium partial ossicular replacement prosthesis served as the model for the design of the 3D-printed prosthesis. 3D models of lengths between 15 and 30 mm were crafted using the SolidWorks 2019-2021 software. TNO155 datasheet Liquid photopolymer Clear V4, in conjunction with vat photopolymerization, was used to manufacture the 3D-printed prostheses.